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Human IL-1α(Interleukin 1 Alpha) ELISA Kit

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ELISA Kits

Human IL-1α(Interleukin 1 Alpha) ELISA Kit

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-1α antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-1α bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-1α in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.:: EH0184
Alias:: Interleukin-1 alpha ELISA Kit , IL-1 alpha ELISA Kit , Hematopoietin-1 ELISA Kit , IL1A ELISA Kit , IL1F1 ELISA Kit
Gene Name:: IL-1α
UniProt ID:: P01583

Manual MSDS Add to Compare

Size	Price
48T/96T	Inquiry

Dispatch Time: About 3 working days

Product Name

Human IL-1α(Interleukin 1 Alpha) ELISA Kit

Catalogue No.

EH0184

Gene Name

IL-1α

UniProt ID

P01583

Alias

Interleukin-1 alpha ELISA Kit , IL-1 alpha ELISA Kit , Hematopoietin-1 ELISA Kit , IL1A ELISA Kit , IL1F1 ELISA Kit

Sample Type

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Method

Sandwich ELISA, Double Antibody

Detection Wavelength

OD450

Reaction Duration

4 hours

Range

3.906-250pg/ml (1pg=160mIU)

Sensitivity

2.344pg/ml

Specificity

Specifically binds with IL-1α , no obvious cross reaction with other analogues.

Storage

2-8°C(Sealed), Don't cryopreserve.

Kit Components

Kit Components	Item	Size(48T)	Size(96T)	Storage Condition for Opened Kit
E001	ELISA Microplate(Dismountable)	8×6	8×12	Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E002	Lyophilized Standard	1vial	2vial	Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E003	Biotin-labeled Antibody(Concentrated, 100X)	60ul	120ul	2-8°C (Avoid Direct Light)
E034	HRP-Streptavidin Conjugate(SABC, 100X)	60ul	120ul
E024	TMB Substrate	5ml	10ml
E039	Sample Dilution Buffer	10ml	20ml	2-8°C
E040	Antibody Dilution Buffer	5ml	10ml
E049	SABC Dilution Buffer	5ml	10ml
E026	Stop Solution	5ml	10ml
E038	Wash Buffer(Concentrated, 25X)	15ml	30ml
E006	Plate Sealer	3 pieces	5 pieces
E007	Product Description	1 copy	1 copy

Typical Standard Curve

STD.(ng/ml)	OD-1	OD-2	Average
0	0.088	0.09	0.089
3.906	0.173	0.179	0.176
7.813	0.276	0.284	0.28
15.625	0.408	0.42	0.414
31.25	0.631	0.649	0.64
62.5	0.921	0.947	0.934
125	1.613	1.659	1.636
250	2.187	2.251	2.219

Precision

Item	Sample	n	Mean (ng/ml)	Standard deviation	CV (%)
Inter-assay Precision	Sample	1	2	3	1
Inter-assay Precision	n	20	20	20	20
Inter-assay Precision	Mean (pg/ml)	7.9	29.67	123.57	7.56
Inter-assay Precision	Standard deviation	0.47	1.29	5.42	0.33
Inter-assay Precision	CV(%)	5.89	4.34	4.39	4.42

Figures for this product are not available in this catalogue.

Storage: 2-8°C(Sealed), Don't cryopreserve.
Dispatch Time: About 3 working days

Ordering & Delivery

How do I place an order?

Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.

What is the dispatch time?

In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.

Do you ship outside Türkiye?

Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.

Storage & Handling

How should an unopened kit be stored?

At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.

How long is an opened kit stable?

It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.

What should I do if the shipment arrives warm?

Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.

Assay Questions

Which sample types can I use?

The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.

My background is too high. What should I check?

The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.

Can I run fewer wells than the kit provides?

Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.

Technical Support

Something did not work. What do you need from me?

Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.

Do you provide a certificate of analysis?

Yes, per lot. Ask for it with the catalogue number and lot number.

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