Products
Human IL-4 (Interleukin 4) ELISA Kit
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EH0199-HS
- Alias:
- Interleukin-4 ELISA Kit , IL-4 ELISA Kit , B-cell stimulatory factor 1 ELISA Kit , BSF-1 ELISA Kit , Binetrakin ELISA Kit , Lymphocyte stimulatory factor 1 ELISA Kit , Pitrakinra ELISA Kit , IL4 ELISA Kit
- Gene Name:
- IL-4
- UniProt ID:
- P05112
- Product Name
- Human IL-4 (Interleukin 4) ELISA Kit
- Catalogue No.
- EH0199-HS
- Gene Name
- IL-4
- UniProt ID
- P05112
- Alias
- Interleukin-4 ELISA Kit , IL-4 ELISA Kit , B-cell stimulatory factor 1 ELISA Kit , BSF-1 ELISA Kit , Binetrakin ELISA Kit , Lymphocyte stimulatory factor 1 ELISA Kit , Pitrakinra ELISA Kit , IL4 ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 3.125-200pg/ml
- Sensitivity
- 1.875pg/ml
- Specificity
- Specifically binds with IL-4 , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.11 0.114 0.112 3.125 0.163 0.167 0.165 6.25 0.196 0.202 0.199 12.5 0.292 0.3 0.296 25 0.471 0.485 0.478 50 0.793 0.815 0.804 100 1.447 1.489 1.468 200 2.102 2.164 2.133 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (pg/ml) 5.88 23.95 98.82 6.06 Inter-assay Precision Standard deviation 0.3 1.26 5.75 0.28 Inter-assay Precision CV(%) 5.02 5.26 5.82 4.68
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
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EH0199-HS — Human IL-4 (Interleukin 4) ELISA Kit