Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit

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This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Smad3/MADH3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Smad3/MADH3 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Smad3/MADH3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
EH2148
Alias: 
Mothers against decapentaplegic homolog 3 ELISA Kit , MAD homolog 3 ELISA Kit , Mad3 ELISA Kit , Mothers against DPP homolog 3 ELISA Kit , hMAD-3 ELISA Kit , JV15-2 ELISA Kit , SMAD family member 3 ELISA Kit , SMAD 3, Smad3, hSMAD3 ELISA Kit , SMAD3 ELISA Kit , MADH3 ELISA Kit
Gene Name: 
Smad3/MADH3
UniProt ID: 
P84022
MSDS
Size Price
48T/96T Inquiry
Dispatch Time: About 3 working days
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Product Name
Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
Catalogue No.
EH2148
Gene Name
Smad3/MADH3
UniProt ID
P84022
Alias
Mothers against decapentaplegic homolog 3 ELISA Kit , MAD homolog 3 ELISA Kit , Mad3 ELISA Kit , Mothers against DPP homolog 3 ELISA Kit , hMAD-3 ELISA Kit , JV15-2 ELISA Kit , SMAD family member 3 ELISA Kit , SMAD 3, Smad3, hSMAD3 ELISA Kit , SMAD3 ELISA Kit , MADH3 ELISA Kit
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
4 hours
Range
0.625-40ng/ml
Sensitivity
0.375ng/ml
Specificity
Specifically binds with Smad3/MADH3 , no obvious cross reaction with other analogues.
Storage
2-8°C(Sealed), Don't cryopreserve.
Kit Components
Kit ComponentsItemSize(48T)Size(96T) Storage Condition for Opened Kit
E001ELISA Microplate(Dismountable) 8×68×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E002Lyophilized Standard 1vial2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E003Biotin-labeled Antibody(Concentrated, 100X) 60ul120ul 2-8°C (Avoid Direct Light)
E034HRP-Streptavidin Conjugate(SABC, 100X) 60ul120ul
E024TMB Substrate 5ml10ml
E039Sample Dilution Buffer 10ml20ml 2-8°C
E040Antibody Dilution Buffer 5ml10ml
E049SABC Dilution Buffer 5ml10ml
E026Stop Solution 5ml10ml
E038Wash Buffer(Concentrated, 25X) 15ml30ml
E006Plate Sealer 3 pieces5 pieces
E007Product Description 1 copy1 copy
Typical Standard Curve
STD.(ng/ml)OD-1OD-2Average
00.0730.0750.074
0.6250.090.0920.091
1.250.1150.1190.117
2.50.2070.2130.21
50.3410.3510.346
100.7830.8050.794
201.361.41.38
402.3842.4542.419
Precision
ItemSamplenMean (ng/ml)Standard deviationCV (%)
Inter-assay Precision Sample1231
Inter-assay Precision n20202020
Inter-assay Precision Mean (ng/ml)1.25.1418.941.24
Inter-assay Precision Standard deviation0.060.330.990.07
Inter-assay Precision CV(%)4.986.435.235.43


Figures for this product are not available in this catalogue.

Storage
2-8°C(Sealed), Don't cryopreserve.
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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