Products
Human HLA-A(Leukocyte Antigen A) ELISA Kit
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HLA-A antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with HLA-A bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HLA-A in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EH3227
- Alias:
- HLA class I histocompatibility antigen, A alpha chain ELISA Kit , Human leukocyte antigen A ELISA Kit , HLA-A ELISA Kit , HLA-A ELISA Kit , HLAA ELISA Kit
- Gene Name:
- HLA-A
- UniProt ID:
- P30443
- Product Name
- Human HLA-A(Leukocyte Antigen A) ELISA Kit
- Catalogue No.
- EH3227
- Gene Name
- HLA-A
- UniProt ID
- P30443
- Alias
- HLA class I histocompatibility antigen, A alpha chain ELISA Kit , Human leukocyte antigen A ELISA Kit , HLA-A ELISA Kit , HLA-A ELISA Kit , HLAA ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 0.313-20ng/ml
- Sensitivity
- 0.188ng/ml
- Specificity
- Specifically binds with HLA-A , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.108 0.114 0.11 0.313 0.18 0.188 0.184 0.625 0.211 0.22 0.216 1.25 0.324 0.337 0.331 2.5 0.543 0.565 0.554 5 0.92 0.957 0.939 10 1.304 1.357 1.33 20 2.245 2.336 2.29 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (ng/ml) 0.66 2.63 10.49 0.63 Inter-assay Precision Standard deviation 0.03 0.13 0.49 0.03 Inter-assay Precision CV(%) 5.23 4.88 4.68 4.88
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
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EH3227 — Human HLA-A(Leukocyte Antigen A) ELISA Kit