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Human Anti-MPV B16R (Monkeypox Virus B16R protein) IgG ELISA Kit
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with Anti-MPV B16R IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti-MPV B16R IgG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EH5774
- Alias:
- MPXV B16R IgG ELISA Kit , Monkeypox virus B16R IgG ELISA Kit
- Gene Name:
- MPV
- Product Name
- Human Anti-MPV B16R (Monkeypox Virus B16R protein) IgG ELISA Kit
- Catalogue No.
- EH5774
- Gene Name
- MPV
- Alias
- MPXV B16R IgG ELISA Kit , Monkeypox virus B16R IgG ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Indirect ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 3 hours
- Range
- 0.781-50ng/ml
- Sensitivity
- 0.469ng/ml
- Specificity
- Specifically binds with Anti-MPV B16R IgG , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 HRP Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.05 0.052 0.051 0.781 0.12 0.124 0.122 1.563 0.24 0.246 0.243 3.125 0.396 0.408 0.402 6.25 0.652 0.67 0.661 12.5 0.933 0.961 0.947 25 1.576 1.622 1.599 50 2.015 2.073 2.044 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (ng/ml) 1.55 6.68 24.98 1.53 Inter-assay Precision Standard deviation 0.09 0.31 1.12 0.08 Inter-assay Precision CV(%) 5.86 4.68 4.49 5.49
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
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EH5774 — Human Anti-MPV B16R (Monkeypox Virus B16R protein) IgG ELISA Kit