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Monkey TGF-α (Transforming Growth Factor α) ELISA Kit
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TGF-α antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TGF-α bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TGF-α in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EMK0324
- Alias:
- TGF-α ELISA Kit , Transforming Growth Factor α ELISA Kit , TGFα ELISA Kit , TGFA ELISA Kit , protransforming growth factor alpha ELISA Kit
- Gene Name:
- TGF-α
- UniProt ID:
- A0A0D9RRL7
- Product Name
- Monkey TGF-α (Transforming Growth Factor α) ELISA Kit
- Catalogue No.
- EMK0324
- Gene Name
- TGF-α
- UniProt ID
- A0A0D9RRL7
- Alias
- TGF-α ELISA Kit , Transforming Growth Factor α ELISA Kit , TGFα ELISA Kit , TGFA ELISA Kit , protransforming growth factor alpha ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 15.625-1000pg/ml
- Sensitivity
- 9.375pg/ml
- Specificity
- Specifically binds with TGF-α , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.09 0.092 0.091 15.625 0.176 0.182 0.179 31.25 0.282 0.29 0.286 62.5 0.401 0.413 0.407 125 0.618 0.636 0.627 250 0.967 0.995 0.981 500 1.554 1.6 1.577 1000 2.164 2.226 2.195 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (pg/ml) 31.91 120.4 509 32.14 Inter-assay Precision Standard deviation 1.47 7.07 27.99 1.62 Inter-assay Precision CV(%) 4.6 5.87 5.5 5.05
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
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EMK0324 — Monkey TGF-α (Transforming Growth Factor α) ELISA Kit