Products
Porcine anti-PEG (Polyethylene glycol) IgG ELISA Kit
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with Anti-PEG IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti-PEG IgG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EP0457
- Alias:
- anti-PEG IgG ELISA Kit , anti-PEG (Polyethylene glycol) IgG ELISA Kit
- Gene Name:
- PEG
- Product Name
- Porcine anti-PEG (Polyethylene glycol) IgG ELISA Kit
- Catalogue No.
- EP0457
- Gene Name
- PEG
- Alias
- anti-PEG IgG ELISA Kit , anti-PEG (Polyethylene glycol) IgG ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Indirect ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 3 hours
- Range
- 0.781-50ng/ml
- Sensitivity
- 0.469ng/ml
- Specificity
- Specifically binds with Anti-PEG IgG , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 HRP Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 0.049 0.051 0.05 0.781 0.128 0.132 0.13 1.563 0.23 0.236 0.233 3.125 0.412 0.424 0.418 6.25 0.619 0.637 0.628 12.5 0.916 0.942 0.929 25 1.56 1.606 1.583 50 2.119 2.181 2.15 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (ng/ml) 1.59 6.58 25.6 1.63 Inter-assay Precision Standard deviation 0.08 0.39 1.02 0.08 Inter-assay Precision CV(%) 5.12 5.86 3.98 4.83
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
How do I place an order?
What is the dispatch time?
Do you ship outside Türkiye?
How should an unopened kit be stored?
How long is an opened kit stable?
What should I do if the shipment arrives warm?
Which sample types can I use?
My background is too high. What should I check?
Can I run fewer wells than the kit provides?
Something did not work. What do you need from me?
Do you provide a certificate of analysis?
Aradığınızı bulamadınız mı? Tüm SSS sayfasına bakın veya bize yazın.
Related Products
Product Inquiry
EP0457 — Porcine anti-PEG (Polyethylene glycol) IgG ELISA Kit