Products
EIF4A3 antibody
Category:
- Catalogue No.:
- FNab02718
- Alias:
- Eukaryotic initiation factor 4A-III (eIF-4A-III antibody, eIF4A-III)|ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD box protein 48|Eukaryotic initiation factor 4A-like NUK-34|Eukaryotic translation initiation factor 4A isoform 3|Nuclear matrix protein 265 (NMP 265 antibody, hNMP 265)|Eukaryotic initiation factor 4A-III antibody, N-terminally processed|EIF4A3|DDX48|KIAA0111 antibody
- Gene Name:
- EIF4A3
- UniProt ID:
- P38919
- Product Name
- EIF4A3 antibody
- Catalogue No.
- FNab02718
- Gene Name
- EIF4A3
- UniProt ID
- P38919
- Alias
- Eukaryotic initiation factor 4A-III (eIF-4A-III antibody, eIF4A-III)|ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD box protein 48|Eukaryotic initiation factor 4A-like NUK-34|Eukaryotic translation initiation factor 4A isoform 3|Nuclear matrix protein 265 (NMP 265 antibody, hNMP 265)|Eukaryotic initiation factor 4A-III antibody, N-terminally processed|EIF4A3|DDX48|KIAA0111 antibody
- Isotype
- IgG
- Form
- liquid
- Purification
- Immunogen affinity purified
- Immunogen
- eukaryotic translation initiation factor 4A, isoform 3
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:2000
- Purity
- ≥95% as determined by SDS-PAGE
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
- Background
- ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex(EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay(NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X(and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development.
Figures for this product are not available in this catalogue.
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
- Dispatch Time
- About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.
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FNab02718 — EIF4A3 antibody