NT5E antibody

References #2
Category:
Catalogue No.: 
FNab05878
Alias: 
5'-nucleotidase (5'-NT)|5'-deoxynucleotidase|Ecto-5'-nucleotidase|IMP-specific 5'-nucleotidase|Thymidylate 5'-phosphatase|NT5E|NT5|NTE antibody
Gene Name: 
NT5E
UniProt ID: 
P21589
Size Price
100µg Inquiry
Dispatch Time: About 3 working days
Contact Us
Product Name
NT5E antibody
Catalogue No.
FNab05878
Gene Name
NT5E
UniProt ID
P21589
Alias
5'-nucleotidase (5'-NT)|5'-deoxynucleotidase|Ecto-5'-nucleotidase|IMP-specific 5'-nucleotidase|Thymidylate 5'-phosphatase|NT5E|NT5|NTE antibody
Isotype
IgG
Form
liquid
Purification
Immunogen affinity purified
Immunogen
5'-nucleotidase, ecto(CD73)
Recommended dilution
WB: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa,64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries(CALJA).


Figures for this product are not available in this catalogue.

Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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Cited Publications (2)

BMP-2 Inhibits the Inflammatory Response and Promotes Bone Formation in Rats with Femoral Fracture by Activating the AMPK Signaling Pathway IF: 1

BIOCELL · 2025-12-12

MiR‐22‐3p facilitates bone marrow mesenchymal stem cell osteogenesis and fracture healing through the SOSTDC1‐PI3K/AKT pathway IF: 1.8

International Journal of Experimental Pathology · 2024-07-05

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