Annexin V-FineTest®Violet 450/PI Apoptosis Kit

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Annexin V-FineTest®Violet 450/PI apoptosis detection kit can be used to detect apoptosis in suspension cells and adherent cells. Annexin V is a calcium-dependent phosphatidylserine binding protein with a high affinity for phosphatidylserine PS. Annexin V labelled FineTest®Violet 450 can bind to the membrane of early apoptotic cells by means of the PS exposed outside the cells. Apoptosis can be detected by flow cytometry or fluorescence microscopy. Propidium Iodide (PI) binds specifically to double-stranded DNA and produces strong fluorescence, which is normally unable to penetrate cell membranes. Due to late apoptotic or necrotic cell membrane loss of integrity, PI can enter cells to stain DNA and, when used in combination with Annexin V, distinguish cells at different stages of apoptosis. The following figure shows the apoptosis effect of Jurkat cells induced by camptothecin detected by this kit.

Catalogue No.: 
K138
Size Price
20T/50T/100T Inquiry
Dispatch Time: About 3 working days
Contact Us
Product Name
Annexin V-FineTest®Violet 450/PI Apoptosis Kit
Catalogue No.
K138
Storage
2-8°C for 12 months. Annexin V-FineTest®Violet 450 and propidium iodide need to be stored away from light.
Kit Components
Kit ComponentsItemSize(48T)Size(96T) Storage Condition for Opened Kit
Reagents20T 50T100T
Annexin V-FineTest®Violet 450100μL 250μL500μL
1X Binding Buffer10mL 25mL50mL
Propidium Iodide (PI)200μL 500μL1mL


Figures for this product are not available in this catalogue.

Storage
2-8°C for 12 months. Annexin V-FineTest®Violet 450 and propidium iodide need to be stored away from light.
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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