Mouse IL-12 (Interleukin 12) QuickTest ELISA Kit

Category:

This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains IL-12, a capture antibody-IL-12-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of IL-12 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
QT-EM1528
Alias: 
IL-12 ELISA Kit , Interleukin-12 subunit alpha ELISA Kit , IL-12A ELISA Kit , Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit , CLMF p35 ELISA Kit , IL-12 subunit p35 ELISA Kit , NK cell stimulatory factor chain 1 ELISA Kit , NKSF1 ELISA Kit , IL12A ELISA Kit , NKSF1 ELISA Kit , Interleukin-12 subunit beta ELISA Kit , IL-12B ELISA Kit , Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit , CLMF p40 ELISA Kit , IL-12 subunit p40 ELISA Kit , NK cell stimulatory factor chain 2 ELISA Kit , NKSF2 ELISA Kit , IL12B ELISA Kit , NKSF2 ELISA Kit , IL-12p70 ELISA Kit
Gene Name: 
IL-12
UniProt ID: 
P43432&P43431
MSDS
Size Price
48T/96T Inquiry
Dispatch Time: About 3 working days
Contact Us
Product Name
Mouse IL-12 (Interleukin 12) QuickTest ELISA Kit
Catalogue No.
QT-EM1528
Gene Name
IL-12
UniProt ID
P43432&P43431
Alias
IL-12 ELISA Kit , Interleukin-12 subunit alpha ELISA Kit , IL-12A ELISA Kit , Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit , CLMF p35 ELISA Kit , IL-12 subunit p35 ELISA Kit , NK cell stimulatory factor chain 1 ELISA Kit , NKSF1 ELISA Kit , IL12A ELISA Kit , NKSF1 ELISA Kit , Interleukin-12 subunit beta ELISA Kit , IL-12B ELISA Kit , Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit , CLMF p40 ELISA Kit , IL-12 subunit p40 ELISA Kit , NK cell stimulatory factor chain 2 ELISA Kit , NKSF2 ELISA Kit , IL12B ELISA Kit , NKSF2 ELISA Kit , IL-12p70 ELISA Kit
Sample Type
Serum, plasma, cell culture supernatant, cell lysate or tissue lysate, other biological fluid samples
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
120 minutes
Range
7.813-500pg/ml
Sensitivity
4.688pg/ml
Specificity
Specifically binds with IL-12 , no obvious cross reaction with other analogues.
Storage
2-8°C(Sealed), Don't cryopreserve.
Kit Components
Kit ComponentsItemSize(48T)Size(96T) Storage Condition for Opened Kit
E001ELISA Microplate(Dismountable) 8×68×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E002Lyophilized Standard 1vial2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E054Cap/Det Ab (Ready to use, blue) 3ml6ml 2-8°C (Avoid Direct Light)
E053HRP-Streptavidin (Ready to use, orange) 5ml10ml
E024TMB Substrate 5ml10ml
E039Sample Dilution Buffer 20ml20ml 2-8°C
E026Stop Solution 5ml5ml
E038Wash Buffer(25X) 15ml30ml
E006Plate Sealer 3 pieces5 pieces
E007Product Description 1 copy1 copy
Typical Standard Curve
STD.(ng/ml)OD-1OD-2Average
00.0970.0990.098
7.8130.1710.1750.173
15.6250.2520.260.256
31.250.3920.4040.398
62.50.6410.6590.65
1250.9240.950.937
2501.5011.5451.523
5001.9712.0292
Precision
ItemSamplenMean (ng/ml)Standard deviationCV (%)
Inter-assay Precision Sample1231
Inter-assay Precision n20202020
Inter-assay Precision Mean (pg/ml)15.2160.4241.615.64
Inter-assay Precision Standard deviation0.833.1612.80.87
Inter-assay Precision CV(%)5.445.235.35.54


Figures for this product are not available in this catalogue.

Storage
2-8°C(Sealed), Don't cryopreserve.
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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